Need of Culture Independent Method for
The Isolation of Novel Actinomycetes from Cave Environment
Abstract
In this article, we reviewed the need of culture independent methods for novel Actinomycetes taxonomy study from cave environments. Because, this hypogean environment could be the main source for the rare strains as well as its secondary metabolites with new chemical entities, particularly variety of new drug production which has more industrial importance. Recent cave studies proved the role of biotechnological methods in the rare actinomycete isolation study. 16s rRNA sequencing method simplifies the determination of this extreme environment living microbial groups. Same time the use of specific and Universal forward and reverse primers also reviewed. However, unfavorable conditions present in cave create stress to actinomycete at genetic level. This paved the way to new species evaluation and its novel metabolites has application on biotechnological industries, particularly antimicrobial activity and biodegradation.
Key Words: cave resource, novel actinomycete, 16s rRNA sequencing
Authors : *Baby Joseph and Sankarganesh
* For Correspondence : E mail : petercmibtiru@gmail.com
