Identification and Process Development for enhanced Production of Lipstatin from Streptomyces toxytricini (Nrrl 15443) and furtherDownstream Processing
Maushmi S. Kumar. Verma,V. Soni, S. and Rao A.S.P
Adv Bio Tech, vol.11, no.7, 06-11, January 2012
ABSTRACT
The selected strain Streptomyces toxytricini (NRRL 15443) was evaluated for production of an anti-obesity drug, Lipstatin. The study includes the optimization of seed media and using Plackett-Burman method for Production fermentation media for increasing the yield of the strain selected. Two extraction methods were used for comparative study followed by purification on silica gel column and identification of lipstatin molecule using sophisticated techniques such as IR, NMR, and mass spectroscopy. The presence and identification was also confirmed TLC and HPLC method. This study also compared the impurity profile & the percentage purity of the lipstatin. The optimized media gave a titer of Lipstatin (1980 mg/ml) which is comparable to highest producing benchmarks (2400 mg/ml), but it is quite economical in a way that this work eliminates the use of L-Leucine as media component. The further increase in yield can be tried by different UV, chemical mutation methods in future.
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Author Affiliations
Maushmi S. Kumar1*, Verma.V1, Soni S2 and Rao A.S.P2
1.Shobhaben Pratapbhai School of Pharmacy and Technology Management, NMIMS Deemed University, Vile Parle (w), Mumbai, India
2.Microbial Process Development Laboratory, Alembic Ltd., Gorwa Road, Vadodara, Mumbai, India
*Corresponding Author E mail : maushmiskumar@gmail.com
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Citation: Maushmi S Kumar, Verma .V, Soni .S and Rao A.S.P, 2012. Identification and Process Development for enhanced Production of Lipstatin
from Streptomyces Toxytricini (Nrrl 15443) and further Downstream Processing. Adv Bio Tech: 11(7): 06-11
