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Abstract

Comparative study on efficiency of genomic DNA Extraction methods in edible mushrooms

Kabilan  M, Arun, B. Saraswathy, N, Ramalingam P.Rajasekaran.

Advanced BioTech, Volume:8 Issue:08 Februry 10, 2009
Abstract
Introduction
White-rot fungi are a class of lignolytic basidiomycetes, capable of degrading lignin in wood. These fungi find their role in bioremediation, bioleaching and numerous other biotechnological applications (Bezalel et al.,1997). Pleurotus ostreatus is an edible basidiomycete of increasing biotechnological interest due to its ability to degrade both chemicals and wood related to lignin degradation products. Furthermore, this fungus produces secondary metabolites with pharmaceutical applications and some proteins of potential industrial use (Bobek et al.1993, Bobek and Ozdin 1994). Agaricus bisporus is also a commercially important edible mushroom. It is a secondary homothallic mushroom with intramictic breeding behavior and low level of genetic recombination (Raper, 1972, Fredricks, 2005). This cultivated species accounts for 90% of mushroom production in India and is a potential indoor crop for rural livelihood and health food. For molecular characterization of these economically important fungi, efficient genomic DNA extraction procedure is essential. Isolation of genomic DNA is an important step in any molecular biology technique. Even though many techniques are available for the isolation of genomic DNA, all have a common outline. The first step in the isolation of the genomic DNA is to disrupt the cellwall to release the cellular contents. Detergents like SDS or CTAB are used to denature the proteins. Other contaminants like  polysaccharides, fatty acids, cellwall materials are removed by precipitation followed by centrifugation. The DNA released in the supernatant is precipitated with ethanol or iso-propanol. The genomic DNA isolation methods used for plants are also employed to isolate DNA from  fungi with little modifications (Giardina et al. 1995). The efficiency of different methods used for the isolation  of DNA varies for different species of mushroom ( Zhang et al., 1996,Muller et al., 1998 and Melo et al., 1998) . The present study aims to find  a suitable method for the isolation of genomic DNA from edible mushrooms.
 
Author Affiliations
 
Kabilan  M, Arun, B. Saraswathy, N, Ramalingam P.Rajasekaran.
Department of Biotechnology, Kumaraguru College of Technology, Coimbatore
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