Isolation and Characterization of Protease Production
of Moderately Halophilic Protease from Newly
Isolated Bacillus aquimaris Strain AP.MSU5
Abstract
The optimum conditions for the protease production by Bacillus aquimaris were found to be at pH 7.0 (230.97 U/ml) and temperature (136.48 U/ml) at 40ºC. The effect of carbon and nitrogen sources on protease production indicated that (179.32 U/ml) maltose and casein (172.18 U/ml) were the apt substrates for protease production respectively. In experimentation with metal ions, the maximum protease production was observed (350.03 U/ml) in the media supplemented with calcium chloride. The halotolerancy of B. aquimaris AP.MSU5 for protease production indicated that, 0.5% sodium chloride was optimum for maximizing the protease production (131.02 U/ml). Among the tested surfactants, the maximum protease production was observed in Tween 20 (154.25 ml) when compared to the other tested surfactants. Effect of hydrocarbons inferred that the maximum protease production was recorded in the nonane (123.88 U/ml) supplemented medium.
Key words: Protease; Bacillus aquimaris AP.MSU5; Optimization; 16S rRNA Gene sequence
Authors : *S.Sankaralingam1, P.Kameshpandiyan2, A.Palavesam2, K.Lingakumar1, D.Prabu1 and V.Thirumalaiarasu1
* For Correspondence : E mail : biosankaralingam@yahoo.co.in
