Vectors Used in Gene Manipulation-
A Retrospective
Abstract
“A vector is a DNA molecule that is capable of replication in a host organism, and can act as a carrier molecule for the transfer of genes into the host. The biology of gene cloning is concerned with the selection and use of a suitable vector, a living system or host in which the vector can be propagated. Based on literature survey a general view is given on vectors used in gene manipulation. Cloning vectors like plasmid, l (insertion) vector, l (replacement) vector, cosmid, phagemids, M13 vector, YAC, BAC and PAC are mainly focused. Expression vector and shuttle vector are also reviewed. Vectors for cloning in higher plants like Ti plasmids, Ri plasmids and vectors for animals cells like SV40, adenovirus, bovine papilloma virus, pox viral vectors are also included. This communication will be helpful for the reader working with different types of vectors used in gene cloning and expression.
Key words: Vector, cloning vectors, expression vectors, vectors for plants and animals.
Author : K.H. Khan
For Correspondence : E mail : hamkishwar191@yahoo.co.in, kishwar@vit.ac.in
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